Seven different (hypo-)allergenic constructs are proposed in developing a construct for peach SIT. wt and rPru p 3 (sequence shown), a "natural hypoallergenic" rFra a 3 (changes in sequence compared to Pru p 3 are underlined), rPru p 3 sur: a surface mutant (3 amino acids mutated to Alanin), rPru p 3 cys: 4 cysteines mutated to serine, rPru p 3 G: glutaraldehyde treated rPru p 3 (involves free amino-groups, indicated), rPru p 3 RA: reduced and alkylated rPru p 3 (involves all cysteines). The known IgE-binding sites of Pru p 3 are boxed, H1-4 indicate the α-helices.
Zuidmeer-Jongejan et al. Clinical and Translational Allergy 2012 2:5 doi:10.1186/2045-7022-2-5